Molecular Protocols in Transfusion Medicine - Softcover

Denomme, Gregory A.

 
9780122093708: Molecular Protocols in Transfusion Medicine

Inhaltsangabe

This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines.

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Über die Autorin bzw. den Autor

Charlotte y Peter Fiell son dos autoridades en historia, teoría y crítica del diseño y han escrito más de sesenta libros sobre la materia, muchos de los cuales se han convertido en éxitos de ventas. También han impartido conferencias y cursos como profesores invitados, han comisariado exposiciones y asesorado a fabricantes, museos, salas de subastas y grandes coleccionistas privados de todo el mundo. Los Fiell han escrito numerosos libros para TASCHEN, entre los que se incluyen 1000 Chairs, Diseño del siglo XX, El diseño industrial de la A a la Z, Scandinavian Design y Diseño del siglo XXI.

Von der hinteren Coverseite

Molecular technology has transformed the practice of laboratory medicine and revolutionized patient care. Molecular Protocols in Transfusion Medicine is a much-needed reference for the transfusion laboratory. If you phenotype blood, perform prenatal serology, specialize in complex antibody investigations, or diagnose neonatal cytopenic disorders, this manual is a must for state-of-the-art analyses of blood group polymorphisms.
The manual provides comprehensive coverage for the molecular bases of polymorphisms expressed on red cells, platelets and neutrophils in a 'facts sheet' format. It covers general concepts in molecular biology and includes specific protocols such as specimen processing, DNA extraction, quality control, PCR set-up, and troubleshooting guides. Other useful adjunct including web site addresses, glossary, and worksheets are also detailed as a guide for the user.
Easy to use, practical and focused, Molecular Protocols in Transfusion Medicine is essential for molecular biologists, scientists, immunohematologists and hematologists involved in the study of human genetic polymorphisms.
Key Features
* Organized by blood group system
* Online references for nucleic acid sequences
* Protocols that allow investigators to set up assays with minimal effort, e.g. primers, reagents, and reaction conditions
* Relevant safety information
* Application and interpretation of results in a variety of clinical settings
* Key references, accession numbers, web sites, request forms, work sheets and glossary
* Clearly presented data complemented by excellent diagrams and tabular material|Molecular technology has transformed the practice of laboratory medicine and revolutionized patient care.Molecular Protocols in Transfusion Medicine is a much-needed reference for the transfusion laboratory. If you phenotype blood, perform prenatal serology, specialize in complex antibody investigations, or diagnose neonatal cytopenic disorders, this manual is a must for state-of-the-art analyses of blood group polymorphisms.
The manual provides comprehensive coverage for the molecular bases of polymorphisms expressed on red cells, platelets and neutrophils in a 'facts sheet' format. It covers general concepts in molecular biology and includes specific protocols such as specimen processing, DNA extraction, quality control, PCR set-up, and troubleshooting guides. Other useful adjunct including web site addresses, glossary, and worksheets are also detailed as a guide for the user.
Easy to use, practical and focused, Molecular Protocols in Transfusion Medicine is essential for molecular biologists, scientists, immunohematologists and hematologists involved in the study of human genetic polymorphisms.
Key Features
* Organized by blood group system
* Online references for nucleic acid sequences
* Protocols that allow investigators to set up assays with minimal effort, e.g. primers, reagents, and reaction conditions
* Relevant safety information
* Application and interpretation of results in a variety of clinical settings
* Key references, accession numbers, web sites, request forms, work sheets and glossary
* Clearly presented data complemented by excellent diagrams and tabular material

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