This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.
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Each of the protocols is written relatively independent of the rest of the text, allowing the reader to immediately begin using a procedure without having to study the text from cover to cover... Very few other molecular biology guide books are available that cover the isolation and manipulation of RNA as exhaustingly as does this one. This book would be highly valuable in any research facility engaged in molecular biology. --Mark A. Jandreski, Loyola University Medical Center, DOODY'S ELECTRONIC JOURNAL
This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.
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