Correlative Light and Electron MIcroscopy: Volume 111 (Methods in Cell Biology) - Hardcover

Hardcover

ISBN 10: 0124160263 ISBN 13: 9780124160262

Verlag: Academic Press, 2012

Inhaltsangabe

Book by None

Über diesen Titel

Reseña del editor:

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

• Covers many areas of CLEM by the best international scientists in the field
• Includes a brief history and overview on CLEM methods

Biografía del autor:

Dr. Thomas Müller-Reichert is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in meiosis, mitosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in the early embryo of the nematode Caenorhabditis elegans and in tissue culture cells. He got his PhD degree from the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards to the EMBL in Heidelberg (Germany) for a post-doc with Dr. Tony Hyman. He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA) and set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is head of the Core Facility Cellular Imaging (CFCI) of the Medical Faculty Carl Gustav Carus of the TU Dresden (Germany).

Together with Dr. Paul Verkade he has developed a rapid transfer system for high-pressure freezing used for Correlative Light and Electron Microcopy. He has organized a number microscopy conferences and taught in several (CL)EM courses. He edited an MCB volume on the Electron Microscopy of Model Systems.

Über diese Auflage

• Verlag: Academic Press
• Erscheinungsdatum: 2012
• ISBN 10: 0124160263
• ISBN 13: 9780124160262
• Einband: Tapa dura
• Anzahl der Seiten: 460
• Herausgeber: Muller-Reichert Thomas, Verkade Paul