While the theoretical basis of centrifugal separations remain the same, recent years have seen dramatic changes in both the design of centrifuges and the range of people that use them. This book is designed to reflect these changing uses of preparative centrifuges by providing detailed protocols covering all of the different types of separation from DNA to cells. Guidelines are also given to help the reader devise new types of separation protocols. The book includes program source code for calculation and simulation programs that are invaluable for quantitive centrifugation methods. In addition, this volume also contains extensive appendices of valuable data that are required by everyone using centrifuges as part of their research work. Preparative Centrifugation: A Practical Approach contains a wealth of guidelines, protocols, and practical advice that will be of direct use to experienced researchers and novices alike in virtually every area of biological research.
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