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Transcription Factors: A Practical Approach - Hardcover

Latchman, David S.

 
9780199633418: Transcription Factors: A Practical Approach

Inhaltsangabe

The pivotal role played by transcription factors in regulating gene expression has given rise to much research and progress in this area. This volume provides detailed practical guidance for identifying, isolating, and characterizing transcription factors. Starting with a specific DNA sequence, these protocols allow the detection and identification of proteins that bind to it and detailed analysis of the DNA binding site. This is followed by procedures for the functional assay of transcription factors and their purification. Also included are methods for the characterization of transcription factor genes, including DNA cloning. The analysis of transcription factors is of central interest to molecular biologists studying gene expression, and this text should be of use to post-graduate students and established researchers alike.

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