RNA Processing: v.2: A Practical Approach - Hardcover

Higgins, Stephen J.

 
9780199634712: RNA Processing: v.2: A Practical Approach

Inhaltsangabe

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.

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Reseña del editor

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.

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Weitere beliebte Ausgaben desselben Titels

9780199634705: RNA Processing: A Practical Approach: RNA Processing: a practical approach: Volume II: v.2 (Practical Approach Series)

Vorgestellte Ausgabe

ISBN 10:  019963470X ISBN 13:  9780199634705
Verlag: IRL Press, 1994
Softcover