C. Elegans: A Practical Approach (Practical Approach Series, Band 213) - Hardcover

Hardcover

ISBN 10: 0199637393 ISBN 13: 9780199637393

Verlag: Oxford University Press, 2000

Inhaltsangabe

Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, theHox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of its entire genome sequence in 1998. This book contains all the basic information onC. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution; information on how to obtain, grow, and maintainC. elegans for use as a model system; the genome project and how to use genome sequence information by searching the database for homologues using different search methods and then how to analyse the search data; the essential practical details of transformation and common uses for the technique; and how to analyse phenotype by microscopy, including Normaski, fluorescence, 4-dimensional, and electron microscopy. Reverse genetics and strategies for gene inactivation that are known to work inC elegans are also outlined, such as epigenetic inactivation and mutational germ line inactivation. Techniques for studying the neurobiology ofC. elegans are given as well as information on conventional genetics and details of the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. All in all, this is the ideal book for those looking to know anything from applicability to the biology ofC. elegans.

Details zur Ausgabe

• Verlag: Oxford University Press
• Erscheinungsdatum: 2000
• Sprache: Englisch
• ISBN 10: 0199637393
• ISBN 13: 9780199637393
• Einband: Gebundene Ausgabe
• Anzahl der Seiten: 281
• Herausgeber: Hope Ian A.
• Kontakt zum Hersteller: ORIGINAL KAISER; https://corp.oup.com/; ; Via Liguria 4; San Giuliano Milanese ; MI; 20098; Italien