Enzyme Assays: A Practical Approach: 257 (Practical Approach Series) - Softcover

Softcover

ISBN 10: 0199638209 ISBN 13: 9780199638208

Verlag: Oxford University Press, U.S.A., 2002

Inhaltsangabe

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include include experimental protocols covering photometric, radiometric, H.P.L.C., and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Über diesen Titel

Reseña del editor

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.

Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration.

This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Biografía del autor

Robert Eisenthal is in the Department of Biology and Biochemistry, University of Bath, U.K. . Michael Danson is in the Department of Biology and Biochemistry, University of Bath, U.K. .

Details zur Ausgabe

• Verlag: Oxford University Press, U.S.A.
• Erscheinungsdatum: 2002
• Sprache: Englisch
• ISBN 10: 0199638209
• ISBN 13: 9780199638208
• Einband: Tapa blanda
• Auflage: 2
• Anzahl der Seiten: 302
• Herausgeber: Eisenthal Robert
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Penguin Random House Ireland Limited; productsafety@penguin.co.uk; ; Morrison Chambers, 32 Nassau Street; Dublin; Irland