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Laboratory Guide to Biochemistry, Enzymology, and Protein Physical Chemistry: A Study of Aspartate Transcarbamylase - Hardcover

Herve, Guy; Maire, Marc Le; Chabaud, Raymond

 
9780306436390: Laboratory Guide to Biochemistry, Enzymology, and Protein Physical Chemistry: A Study of Aspartate Transcarbamylase
Hardcover
ISBN 10: 0306436396 ISBN 13: 9780306436390
Verlag: Springer, 1991

Inhaltsangabe

1 Aspartate Transcarbamylase.- 2 Molecular Genetics: Regulation of Aspartate Transcarbamylase Biosynthesis.- 3 Purification of Aspartate Transcarbamylase and Its Subunits.- 4 Structural and Physicochemical Study of Aspartate Transcarbamylase.- 5 Enzymatic Catalysis and Regulation.- 6 Complementary Experiments.- 1. Equations and Units.- 2. Molar Mass and Molecular Mass.- 3. Units of Catalytic Activity.- 4. Units of Radioactivity.- 5. Units of Quantity.- 7. Calculation of Acceleration.- 8. Bacterial Strains.- 9. Solutions and Reagents.- 9.1. 0.8-M Tris-Acetate Buffer, pH 8.- 9.2. 0.2-M Acetic Acid.- 9.6. Elution Buffer for Chromatography: 10 × Stock Solution.- 9.7. 100-mM Aspartate, pH 8.- 9.8. 10-mM Carbamylaspartate, pH 8.- 9.9. 1-M Phosphate Buffer, pH 7.2.- 9.10. Buffer for Dilution of E.- 9.11. Buffer for Dilution of C.- 9.12. Buffer for Dilution of R and Recombination.- 9.13. 200-mM Cacodylate Buffer, pH 6, 7, and 7.5.- 9.14. 200-mM Tris-Acetate Buffer, pH 8 and 9.- 9.15. 200-mM Glycine Buffer, pH 10.- 9.16. 400-mM Succinate, pH 7.- 9.17. Reaction Medium: 20-mM CAP-200-mM Tris-Acetate, pH 8.- 10. Preparation of Standard Protein Mixtures for Column Calibration.- Answers To Questions.- References.

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Reseña del editor

This guide for a laboratory course presents an integrated set of experiments relying entirely on the use of unique enzyme, aspartate transcarbamylase, which exhibits all of the catalytic and regulatory properties characteristic of allosteric enzymes. A comprehensive study of this enzyme and its diss

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Verlag
Springer
Erscheinungsdatum
1991
Sprache
Englisch
ISBN 10 
0306436396
ISBN 13 
9780306436390
Einband
Tapa dura
Anzahl der Seiten
184
Kontakt zum Hersteller
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