In Situ Hybridization in Electron Microscopy (Methods in Visualization) - Softcover

Morel, Gerard

Softcover

ISBN 10: 0367455374 ISBN 13: 9780367455378

Verlag: CRC Press, 2020

Inhaltsangabe

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization prior to embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.

The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

Über diesen Titel

Über die Autorin bzw. den Autor

Morel, Gerard | Cavalier, Annie | Williams, Lynda

Details zur Ausgabe

• Verlag: CRC Press
• Erscheinungsdatum: 2020
• Sprache: Englisch
• ISBN 10: 0367455374
• ISBN 13: 9780367455378
• Einband: Taschenbuch
• Anzahl der Seiten: 472
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Buchbär; info@buchbaer.de; ; Weberstr. 67; Osnabrück; 49084; Deutschland