Biocatalysis: Volume 714 (Methods in Enzymology, Volume 714, Band 714) - Hardcover

Hardcover

ISBN 10: 0443317887 ISBN 13: 9780443317880

Verlag: Academic Press, 2025

Inhaltsangabe

Biocatalysis, Volume 714 provides a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Specific chapters in this new release include Biocatalysis: How to select the proper mode of application, Documentation in biocatalysis and data handling, Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applications, Functional Metaproteomics, Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases, P450 monooxygenase in whole-cell format, and more.

Additional sections cover Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cells, Recombinant enzyme expression and targeted mutagenesis in Aromatoleum species, C.necator as a model organism for CO2-based biotechnology, Cupriavidus in whole-cell biocatalysis, Whole-cell biocatalysis with Myxobacteria, Streptomyces for natural product formation: Targeted mutagenesis in PKS, Reductive Amination: Methods for cell-free and whole-cell biocatalysis, Challenges and good practices on transaminase-catalysed synthesis of optically pure amines, W-enzymes in biocatalysis: Chances and difficulties for the user, Atroposelective biocatalysis employing ADHs, Applications of alcohol oxidases, and much more.

Über diesen Titel

Über die Autorin bzw. den Autor

Dirk Tischler studied Applied Natural Science with focal points on Environmental Microbiology and Biotechnology as well as Environmental Analytics at TU Bergakademie Freiberg (Germany) and graduated in 2007. He continued in Freiberg with his doctoral studies on styrene monooxygenases and related enzymes and completed his dissertation with distinction in 2012. For this he was awarded with two pre-doctoral scholarships from the Deutsche Bundesstiftung Umwelt and Fulbright and he had two international research stays in Biochemistry Laboratories with Willem van Berkel at Wageningen University and George Gassner at San Francisco State University. The doctoral thesis was awarded with two prizes. He continued as a group leader in the field of industrial biotechnology with emphasis on the prediction of biocatalysts and pathways from (meta)genomes of soil bacteria, especially actinobacteria. This includes three prestigious grants for his group projects GETGEOWEB (ESF), BakSolEx (BMBF), and ChemBioCat (NRW). 2018 he became W1 Professor and was tenured in 2019 to Full Professor for Microbial Biotechnology at the Ruhr University Bochum. He focuses on the identification and application of novel biocatalysts, mostly related to redox biochemistry.

Von der hinteren Coverseite

The conversion of a substrate into product is a transformation or often also designated as reaction which follows thermodynamic and kinetic boundaries. To drive reactions in a desired direction we employ catalysts with certain properties, and in biocatalysis these are biomolecules, mostly enzymes. Besides enzymes, also proteins, such as antibodies or nucleic acids, can become catalytically active under certain conditions. They all have in common that a three-dimensional structure provides the basis to interact with ligands. In a few cases cofactors, e.g. small organic molecules or ions, contribute to this interface. This interplay results in mostly weak interactions, such as electrostatic (ionic), dipole, hydrogen bonds, charge, transfer, Van der Waals, hydrophobic, and (possible) combinations thereof. In case those interactions sum up to enough power, the ligands are not just bound but changed according to a reaction trajectory to mostly lower energy levels: the transformation into a product can take place. Along this route to the product the activation barrier needs to be overcome, which may be achieved by those mentioned interactions of substrate and catalyst. For biocatalysis this should be possible at elevated velocity compared to non-selective or uncatalyzed processes. Strategies to achieve this can be increasing the chance that substrate and catalyst meet (lowering entropy), lowering surface interactions (e.g., via altered water or solvent shells of substrate and/or catalyst), creating interactions that lead to structural changes and tensions (entatic state), as well as triggering favored orientations for reaction initiation. In addition to the reaction velocity, selectivity is of great importance for the success of the reaction and thus for the user. Biocatalysts can offer various levels of selectivity depending on their three-dimensional structure and the catalyzed reaction: substrate selectivity, defined reaction course, chemo-, regio- and enantioselectivity. Hence, biocatalysts often allow minimizing byproduct formation and at the same time high conversions to a defined product are achievable. All this is defined by the structure of the (bio)catalyst which is written in the genetic code leading to a primary to tertiary or even quaternary structure. Such complex biomolecules bring other properties that require consideration, such as stability (e.g., solvent-, temperature-affected), inhibition and inactivation, and a defined window of operation. The latter is often mentioned as “mild reaction conditions” but means that many biocatalysts must be handled under physiological conditions or need to be stabilized somehow for more harsh applications. However, the genetic information can be altered and thus the biocatalyst can be adjusted on demand. In recent years, the field of protein engineering introduced many tools to improve desired properties to broaden substrate scope, increase stability, define selectivity, introduce new reactions, alter cofactors, among others. In addition, powerful sequencing (meta- and isolated genomic DNA), rapid protein identification, and high-throughput screening methods support the identification of powerful biocatalysts from natures’ reservoir or engineering campaigns. However, the powerful biocatalyst alone does not mean per se that it can be employed in (industrial) processes. Here several other parameters need to be considered, such as substrate availability, biocatalyst performance under process conditions, energy demand, re-usability, single or multiple reaction setups, and product recovery, for example. Thus, the mode of operation is often dictated by the desired reaction in combination with biocatalyst properties and process parameters.

In this volume, the authors provide a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Along this line, the user may encounter various decision points and so guidance on the proper format for a desired application introduces the field and is supported by some advice on documentation for reproducible and fair data handling. Enzyme mining and screening can be done on various levels, too, and it does affect the later application chances as well. Respectively, selected examples for screening and biocatalyst application setups are given herein. Finally, it should be mentioned that processes need to be evaluated and biocatalysis is not always green per se! Hence, the authors hope to provide a supportive resource and some role models for the user to plan and succeed with a biocatalysis project.

Details zur Ausgabe

• Verlag: Academic Press
• Erscheinungsdatum: 2025
• Sprache: Englisch
• ISBN 10: 0443317887
• ISBN 13: 9780443317880
• Einband: Gebundene Ausgabe
• Auflage: 1
• Anzahl der Seiten: 532
• Herausgeber: Tischler
• Kontakt zum Hersteller: preigu GmbH & Co. KG; mail@preigu.de; ; Lengericher Landstr. 19; Osnabrück; Niedersachsen; 49078; Deutschland