Molecular Biology Problem Solver: A Laboratory Guide - Softcover

Softcover

ISBN 10: 0471379727 ISBN 13: 9780471379720

Verlag: WL, 2001

Inhaltsangabe

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.

Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:

• Have you selected the most appropriate research strategy?
• Have you identified the issues critical to your successful application of a technique?
• Are you familiar with the limitations of a given technique?
• When should common procedural rules of thumb not be applied?
• What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:

• Working safely with biological samples and radioactive materials
• DNA and RNA purification
• PCR
• Protein and nucleid acid hybridization
• Prokaryotic and eukaryotic expression systems
• Properly using and maintaining laboratory equipment

Über diesen Titel

Über die Autorin bzw. den Autor

ALAN GERSTEIN and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists.

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Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful application of a technique?
* Are you familiar with the limitations of a given technique?
* When should common procedural rules of thumb not be applied?
* What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
* Working safely with biological samples and radioactive materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment

Details zur Ausgabe

• Verlag: WL
• Erscheinungsdatum: 2001
• Sprache: Englisch
• ISBN 10: 0471379727
• ISBN 13: 9780471379720
• Einband: Taschenbuch
• Auflage: 1
• Anzahl der Seiten: 590
• Herausgeber: Gerstein Alan S.
• Kontakt zum Hersteller: Wiley-VCH GmbH; product_safety@wiley.com; ; Boschstrasse 12; Weinheim; 69469; Deutschland