Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis, 54, Band 54) - Hardcover

Janson, Jan-Christer

Hardcover

ISBN 10: 0471746614 ISBN 13: 9780471746614

Verlag: Wiley, 2011

Inhaltsangabe

This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Ligands from combinatorial libraries
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Two-dimensional electrophoresis in proteomics
* Protein elution and blotting techniques
* Large-scale electrophoresis processes

Über diesen Titel

Über die Autorin bzw. den Autor

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

Von der hinteren Coverseite

The authoritative guide on protein purification now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

• High-resolution reversed-phase liquid chromatography

• Electrophoresis in gels

• Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

• Affinity ligands from chemical and biological combinatorial libraries

• Membrane separations

• Refolding of inclusion body proteins from E. coli

• Purification of PEGylated proteins

• High throughput screening techniques in protein purification

• The history of protein chromatography

Aus dem Klappentext

The authoritative guide on protein purification—now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

• High-resolution reversed-phase liquid chromatography

• Electrophoresis in gels

• Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

• Affinity ligands from chemical and biological combinatorial libraries

• Membrane separations

• Refolding of inclusion body proteins from E. coli

• Purification of PEGylated proteins

• High throughput screening techniques in protein purification

• The history of protein chromatography

Details zur Ausgabe

• Verlag: Wiley
• Erscheinungsdatum: 2011
• Sprache: Englisch
• ISBN 10: 0471746614
• ISBN 13: 9780471746614
• Einband: Gebundene Ausgabe
• Auflage: 3
• Anzahl der Seiten: 532
• Kontakt zum Hersteller: Wiley-VCH GmbH; product_safety@wiley.com; ; Boschstrasse 12; Weinheim; 69469; Deutschland