Insect Cell Cultures: Fundamental and Applied Aspects (Current Applications of Cell Culture Engineering, 2, Band 2) - Hardcover

Hardcover

ISBN 10: 0792334035 ISBN 13: 9780792334033

Verlag: Springer, 1996

Inhaltsangabe

Insect Cell Lines.- Development and characterization of insect cell lines.- New approaches to insect tissue culture.- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene.- Insect cell physiology.- Insect cell cultivation: growth and kinetics.- Medium design for insect cell culture.- Viruses.- Baculovirus-insect cell interactions.- Replication patterns and cytopathology of cells infected with baculoviruses.- Construction of baculovirus recombinants.- Passage effect of virus infection in insect cells.- Post-translational modifications in insect cells.- Productivity of insect cells for recombinant proteins.- Chaperone and foldase coexpression in the baculovirus-insect cell expression system.- Engineering Aspects.- Shear sensitivity of insect cells.- Insect cell bioreactors.- Perfusion bioreactors for the production of recombinant proteins in insect cells.- Immobilization of insect cells.- Modelling baculovirus infection of insect cells in culture.- Scale up aspects of sparged insect-cell bioreactors.- Oxygen gradients in small and big sparged insect-cell bioreactors.- Downstream processing of insect cell cultures.- Applications.- Parvovirus diagnostics and vaccine production in insect cells.- Classical swine fever virus diagnostics and vaccine production in insect cells.- Production of multidomain complement glycoproteins in insect cells.- Economic and Regulatory Aspects.- Economics of baculovirus-insect cell production systems.- Safety aspects of insect cell culture.- Regulatory issues in the use of insect-cell culture.

Details zur Ausgabe

• Verlag: Springer
• Erscheinungsdatum: 1996
• Sprache: Englisch
• ISBN 10: 0792334035
• ISBN 13: 9780792334033
• Einband: Gebundene Ausgabe
• Anzahl der Seiten: 324
• Herausgeber: Vlak Just M., De Gooijer Cornelis D., Tramper Johannes, Miltenburger Herbert G.
• Kontakt zum Hersteller: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Deutschland