In Situ PCR and Related Technology - Hardcover

Hardcover

ISBN 10: 0817638709 ISBN 13: 9780817638702

Verlag: Birkhäuser, 1995

Inhaltsangabe

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Reseña del editor:

Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology. The significance of this objective is obvious. A technique finally materialized in 1990 when Dr. Ashley T. Haase and coworkers published results that used multiple prim­ ers with complementary tails in intact cells. Since then, a number of laboratories have successfully developed their own versions of the technique. In situ peR is now a well-recognized method that permits the detection of minute quantities of DNA or RNA in intact cells or tissue sections. As a result, morphological analysis of those target nucleotide sequences becomes possible. As anticipated, this ad­ vancement has led to significant improvement in our understanding of many nor­ mal and abnormal conditions, and its impact is becoming more evident as time passes. In situ peR has the characteristics of a new landmark in morphologic technol­ ogy-it is scientifically fascinating and technically challenging. In essence, it is a combination of in situ hybridization and conventional peR. The wealth of litera­ ture, experience and protocols for the two latter techniques can be applied to in situ peR. In situ peR also has its own unique aspects that were not addressed by the other two techniques.

Reseña del editor:

"In situ" PCR is capable of detecting minute quantities of DNA or RNA in intact cells or tissues. It provides a useful tool for answering a number of questions that might not otherwise have been investigated. A number of applications of this technology have been reported, bringing advancement in the understanding of many normal and pathalogical conditions, particularly in AIDS and gene therapy research. This work offers a review of the status of this technology, presenting its variations and discussing its applications, including a debate on the theoretical considerations and practical step-by-step protocols.

Über diese Auflage

• Verlag: Birkhäuser
• Erscheinungsdatum: 1995
• ISBN 10: 0817638709
• ISBN 13: 9780817638702
• Einband: Tapa dura
• Anzahl der Seiten: 143
• Herausgeber: Gu Jiang