Basic Methods in Protein Purification and Analysis: A Laboratory Manual - Hardcover

Hardcover

ISBN 10: 0879698683 ISBN 13: 9780879698683

Verlag: Cold Spring Harbor Laboratory Press,U.S., 2008

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A collection of convenient and easy to use, at the bench protocols for protein purification and further manipulations. Some of the methods describing protein purification are from Proteins and Proteomics and Purifying Proteins for Proteomics manuals, with additional information from Protein–Protein Interactions 2e (Standard Technologies).

Reseña del editor:

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

Über diese Auflage

• Verlag: Cold Spring Harbor Laboratory Press,U.S.
• Erscheinungsdatum: 2008
• ISBN 10: 0879698683
• ISBN 13: 9780879698683
• Einband: Tapa dura
• Anzahl der Seiten: 436
• Herausgeber: Simpson Richard J., Adams Peter D., Golemis Erica A.