Research in the field of molecular biology has progressed at a fascinating rate in recent years. Much of this progress results from the development of new laboratory techniques that allow very precise fractionation and analysis of nucleic acids and proteins, as well as the construction of recom binant DNA molecules that can then be cloned and expressed in host cells. Progress has been so rapid that there has been a shortfall in the training of appropriately qualified staff. Many existing laboratory workers require retraining, and many educational institutions have had difficulty incor porating the new molecular biology techniques into their teaching programs. Although there are several manuals currently available that describe laboratory techniques in molecular biology, they are principally written for the indivi dual research worker and are not intended for use in the design of practical classes for students. The aim of this book is to provide just such a series of pro tocols forthe teaching of practical molecular biology. The idea arose following the success of several Workshops in Molecular Biology, organized and taught by staff in the Biol ogy Department of the Hatfield Polytechnic. Gradually, the protocols used in the workshops have been incorporated into the Hatfield undergraduate and postgraduate teaching pro grams and have now been collected together to form a book.
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Soft Cover. Zustand: Very Good. 378pp. Illustrated. Text clean and bright, binding tight, only flaw is a blank bookplate from a chemical company pasted on the front free endpaper." An excellent experimental guide to molecular biology, offering detailed protocols ranging from chemical to microbiological methods. The format is sufficiently versatile to serve either a short workshop or a full academic year biochemistry laboratory. Each of the 25 experiments included is presented in a chapter with background information, a list of materials the experimenter will encounter, a detailed protocol, information needed to interpret and discuss the results, and a few references. Appendix 1 provides general laboratory procedures and safety information; Appendix 2 lists materials and equipment necessary for a group of 20 students working in pairs. The procedures are designed with a high probability of success on the first attempt. The lists of supplies, equipment, and material requiring advance preparation are comprehensive." Among the topics are DNA isolation in E. coli, restriction site mapping, preparative electrophoresis, microisolation of plasmid DNA, messenger RNA, thin-layer gel filtration, Ames test, and more, all providing a well-rounded grounding in molecular biology techniques. Bestandsnummer des Verkäufers S4-000176
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