Basic Cell Culture Protocols (Methods in Molecular Biology, 75)

ISBN 10: 0896033848 ISBN 13: 9780896033849

Verlag: Humana, 1997

Inhaltsangabe

It is now seven years since the original Animal Cell Culture volume was published. During this time, techniques have advanced dramatically. The- fore, we have completely revised this edition, including many new chapters and updating old chapters. There has also been a proliferation of methods books covering specific areas of tissue culture. Thus, in this revised edition, we have left out chapters on hybridoma technology because of the many books dealing specifically with this subject. Instead, we have concentrated on the original purpose of this volume now entitled Basic Cell Culture Protocols- which was to provide easy-to-follow protocols for animal cell culture, that hopefully, will be useful to experts and beginners alike. We would like again to acknowledge Dr. Cliff Stanners who taught me (JWP) cell culture, as well as members of the Toronto Somatic Cell Genetics Group for their advice and encouragement, in particular, Drs. L. Siminovitch and V. Ling. Elyse Rizzo's valuable secretarial assistance in compiling this new volume is greatly appreciated. Jeffrey Pollard John M. Walker W. Contents Preface ............................................................................................................. v Contributors ..................................................................................................... xi 1 Basic Cell Culture Jeffrey Pollard .................................................................................. 1 2 Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts Gareth Jones and Clare J. Wise ..................................................

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Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its distinguished contributors-often the perfectors of the detailed techniques being described-provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy.

Basic Cell Culture Protocols, 2nd ed. will surely follow in the footsteps of the first edition and, in the words of the reviewers, prove a still more "clear, easy-to-use well-sourced, and precise" work that belongs "in every cell culture laboratory."

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 1997
• Sprache: Englisch
• ISBN 10: 0896033848
• ISBN 13: 9780896033849
• Einband: Spiralbindung
• Auflage: 2
• Anzahl der Seiten: 504
• Herausgeber: Pollard Jeffrey W., Walker John M.
• Kontakt zum Hersteller: Nicht verfügbar
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