Transmembrane Signaling Protocols (Methods in Molecular Biology, 84, Band 84)

ISBN 10: 0896034321 ISBN 13: 9780896034327

Verlag: Humana, 1998

Inhaltsangabe

Progress within any scientific discipline rests firmly on the development of methodologies that afford new ways to address unsolved questions. In the past decade, the development and application of novel technologies and approaches for structure determination, biochemical characterization, and molecular manipulation of signaling molecules have resulted in remarkable progress in the understanding of signal transduction pathways. Thus, whereas not so long ago small molecules known as second messengers were the only identified mediators of transmembrane signals, it is now clear that many (per­ haps most) signal transduction pathways operate through complex networks of protein-protein interactions. In recognition of this central mechanism, Transmembrane Signaling Pro­ tocols is largely devoted to techniques that are widely used to study molecular crosstalk between signaling molecules. The two underlying goals of the chap­ ters assembled in this volume are: to offer technical information that allows reproduction of these methods and to provide the general principles on which these are based. In addition, introductory reviews have been included to fur­ nish a comprehensive overview of recent advances in fundamental areas of cell signaling, and to relate specific techniques to the larger picture. I thank the many authors who spent time and effort to contribute to this endeavor. It is hoped that Transmembrane Signaling Protocols will serve as a resource for future progress in the rapidly expanding field of signal transduction.

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Transmembrane Signaling Protocols brings together a collection of practical, cutting-edge techniques for the study of cell signaling. Written by acknowledged leaders in the field, the protocols provide detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the latest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols.

The time-tested techniques collected in Transmembrane Signaling Protocols make available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. The book is destined to become a standard resource for both conceptual information and the practical detail needed to carry out and interpret laboratory procedures in this dramatically advancing field.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 1998
• Sprache: Englisch
• ISBN 10: 0896034321
• ISBN 13: 9780896034327
• Einband: Spiralbindung
• Auflage: 1
• Anzahl der Seiten: 341
• Herausgeber: Bar-Sagi Dafna
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: HUMANA SPAIN S.L.; https://www.humana-spain.org/?lang=en; ; Av Alberto Alcocer 46, 5-B; Madrid; 28016; Spanien