Pcr Protocols: v. 226 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896036421 ISBN 13: 9780896036420

Verlag: Humana Press Inc., 2003

Inhaltsangabe

Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expanded its use in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The meethods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work.

Über diesen Titel

Críticas

Reviews of the First Edition: "...succeeds in covering a wider range of more general applicable methods than...its predecessors ...provid[es] an extensive range of versatile, expedient, and readily applicable PCR protocols."-Trends in Cell Biology From reviews of the first edition... "...useful in virtually all disciplines of the life sciences...a pleasure [to] read and use..." -American Journal of Physiology: Lung Cellular and Molecular Physiology "...an excellent resource...has potential value for anyone looking for new ways to use PCR or new tricks to make their PCR work better." -Biopharm "...provides an extensive range of versatile, expedient, and readily applicable PCR protocols." -Trends in Cell Biology "It is what all molecular biology laboratories should have and contains information that is relevant to all levels of researchers. Relevant and timely diagrams compliment the text; there are also heaps of references included at the end of each section." - Microbiologist

Reseña del editor

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Details zur Ausgabe

• Verlag: Humana Press Inc.
• Erscheinungsdatum: 2003
• Sprache: Englisch
• ISBN 10: 0896036421
• ISBN 13: 9780896036420
• Einband: Tapa dura
• Anzahl der Seiten: 556
• Herausgeber: Bartlett John M. S., Stirling David
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Nicht verfügbar