In Situ Hybridization Protocols (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896036863 ISBN 13: 9780896036864

Verlag: Humana Press Inc., 1999

Inhaltsangabe

Ian Darby updates the highly successful premier edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques - many not covered in the first edition - and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.

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InIn Situ Hybridization Protocols, 2nd Edition, Ian Darby updates the highly successful 1st edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques-many not covered in the first edition-and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Each protocol provides detailed, easy-to-follow instructions, along with realistic commentaries on how to vary their application and how to troubleshoot problems that arise.
Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire .

Details zur Ausgabe

• Verlag: Humana Press Inc.
• Erscheinungsdatum: 1999
• Sprache: Englisch
• ISBN 10: 0896036863
• ISBN 13: 9780896036864
• Einband: Gebundene Ausgabe
• Anzahl der Seiten: 343
• Herausgeber: Darby Ian A.
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: HUMANA SPAIN S.L.; https://www.humana-spain.org/?lang=en; ; Av Alberto Alcocer 46, 5-B; Madrid; 28016; Spanien