Cell-Free Gene Expression: Methods and Protocols (Methods in Molecular Biology, Band 2433) - Softcover

 
9781071620007: Cell-Free Gene Expression: Methods and Protocols (Methods in Molecular Biology, Band 2433)
Softcover
ISBN 10: 1071620002 ISBN 13: 9781071620007
Verlag: Humana, 2023

Inhaltsangabe

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.

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Von der hinteren Coverseite

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.

„Über diesen Titel“ kann sich auf eine andere Ausgabe dieses Titels beziehen.

Verlag
Humana
Erscheinungsdatum
2023
Sprache
Englisch
ISBN 10 
1071620002
ISBN 13 
9781071620007
Einband
Taschenbuch
Auflage
1
Anzahl der Seiten
456
Herausgeber
Karim Ashty S., Jewett Michael C.
Kontakt zum Hersteller
Springer-Verlag GmbH
customerservice@springernature.com

Tiergartenstraße 17
Heidelberg
Baden-Württemberg
69121
Deutschland

Weitere beliebte Ausgaben desselben Titels

9781071619971: Cell-Free Gene Expression: Methods and Protocols (Methods in Molecular Biology, 2433, Band 2433)

Vorgestellte Ausgabe

ISBN 10:  1071619977 ISBN 13:  9781071619971
Verlag: Humana, 2022
Hardcover