Directed Evolution: Methods and Protocols: 2461 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 1071621513 ISBN 13: 9781071621516

Verlag: Springer-Verlag New York Inc., 2022

Inhaltsangabe

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

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This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful <i>Methods in Molecular Biology</i> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.&nbsp;<br><div>Cutting-edge and comprehensive, <i>Directed Evolution: Methods and Protocols</i> is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.</div><div><br></div>

Details zur Ausgabe

• Verlag: Springer-Verlag New York Inc.
• Erscheinungsdatum: 2022
• Sprache: Englisch
• ISBN 10: 1071621513
• ISBN 13: 9781071621516
• Einband: Tapa dura
• Anzahl der Seiten: 336
• Herausgeber: Currin Andrew, Swainston Neil
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Nicht verfügbar