Digital PCR: Methods and Protocols (Methods in Molecular Biology, 2969, Band 2969) - Hardcover

Hardcover

ISBN 10: 1071647660 ISBN 13: 9781071647660

Verlag: Humana, 2025

Inhaltsangabe

This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of uses, including fundamental research, preclinical studies, human applications, and regulatory contexts. Additionally, protocols that further extend potential applications, such as protein detection, the development of custom dPCR systems, and alternative PCR strategies like hpPCR, which, while not digital, are relevant in similar contexts, are covered. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Digital PCR: Methods and Protocols serves as an ideal guide to this mature technology that will continue to enhance target quantification, notably through its multiplexing capabilities, tolerance to inhibitors, and increased precision.

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This detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of uses, including fundamental research, preclinical studies, human applications, and regulatory contexts. Additionally, protocols that further extend potential applications, such as protein detection, the development of custom dPCR systems, and alternative PCR strategies like hpPCR, which, while not digital, are relevant in similar contexts, are covered. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Digital PCR: Methods and Protocols serves as an ideal guide to this mature technology that will continue to enhance target quantification, notably through its multiplexing capabilities, tolerance to inhibitors, and increased precision.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2025
• Sprache: Englisch
• ISBN 10: 1071647660
• ISBN 13: 9781071647660
• Einband: Gebundene Ausgabe
• Anzahl der Seiten: 299
• Herausgeber: Pavlovic Guillaume
• Kontakt zum Hersteller: preigu GmbH & Co. KG; mail@preigu.de; ; Lengericher Landstr. 19; Osnabrück; Niedersachsen; 49078; Deutschland