Plant Immunity: Methods and Protocols (Methods in Molecular Biology, 3012) - Hardcover

Hardcover

ISBN 10: 1071651374 ISBN 13: 9781071651377

Verlag: Humana, 2026

Inhaltsangabe

This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Immunity: Methods and Protocols serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.

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This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Immunity: Methods and Protocols serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2026
• Sprache: Englisch
• ISBN 10: 1071651374
• ISBN 13: 9781071651377
• Einband: Gebundene Ausgabe
• Anzahl der Seiten: 277
• Herausgeber: Kawasaki Tsutomu, Yoshioka Hirofumi
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland