Methods of Protein Microcharacterization: A Practical Handbook (Biological Methods) - Softcover

Shively, John E. E.

Softcover

ISBN 10: 1489941320 ISBN 13: 9781489941329

Verlag: Humana, 2013

Inhaltsangabe

Microisolation Techniques.- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography.- Reverse-Phase HPLC Isolation and Microsequence Analysis.- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis.- Microamino Acid Analysis.- Amino Acid Analysis With Postcolumn Fluorescent Derivatization.- Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC.- Amino Terminal Analysis.- Manual Edman Sequencing System.- Comparison of the Spinning Cup and Gas-Phase Instruments.- Gas-Phase Protein/Peptide Sequencer.- Design of a Multipurpose Sequencer.- Solid-Phase Methods in Protein Microsequence Analysis.- Analysis of Phenylthiohydantoin Amino Acids by HPLC.- Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation.- Carboxyl-Terminal Analysis.- Microsequence Analysis by Enzymatic Methods.- COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods.-Mass Spectrometric Analysis of Polypeptides.- Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry.- Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2013
• Sprache: Englisch
• ISBN 10: 1489941320
• ISBN 13: 9781489941329
• Einband: Taschenbuch
• Anzahl der Seiten: 480
• Kontakt zum Hersteller: Springer Heidelberg; buchhandel-buch@springer.com; ; Tiergartenstr. 17; Heidelberg; 69121; Deutschland