TALENs: Methods and Protocols (Methods in Molecular Biology, 1338, Band 1338) - Hardcover

Hardcover

ISBN 10: 1493929313 ISBN 13: 9781493929313

Verlag: Humana, 2015

Inhaltsangabe

​This volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2015
• Sprache: Englisch
• ISBN 10: 1493929313
• ISBN 13: 9781493929313
• Einband: Gebundene Ausgabe
• Auflage: 1
• Anzahl der Seiten: 286
• Herausgeber: Kühn Ralf, Wurst Wolfgang, Wefers Benedikt
• Kontakt zum Hersteller: Springer-Verlag GmbH; customerservice@springernature.com; ; Tiergartenstraße 17; Heidelberg; Baden-Württemberg; 69121; Deutschland