Nucleic Acid Crystallography: Methods and Protocols (Methods in Molecular Biology, Band 1320) - Softcover

Softcover

ISBN 10: 1493946412 ISBN 13: 9781493946419

Verlag: Humana, 2016

Inhaltsangabe

This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.

Über diesen Titel

Über die Autorin bzw. den Autor

Eric Ennifar Institut de Biologie Moléculaire et Cellulaire du CNRS 15 Rue René Descartes F-67084 Strasbourg, France +33 (0)3 88 41 70 02 email: e.ennifar@ibmc-cnrs.unistra.fr.

Von der hinteren Coverseite

This volume includes comprehensive and up-to-date coverage of all nucleic acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2016
• Sprache: Englisch
• ISBN 10: 1493946412
• ISBN 13: 9781493946419
• Einband: Taschenbuch
• Anzahl der Seiten: 368
• Herausgeber: Ennifar Eric
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland