The Genetic Manipulation of Staphylococci: Methods and Protocols (Methods in Molecular Biology, Band 1373) - Softcover

Softcover

ISBN 10: 1493949004 ISBN 13: 9781493949007

Verlag: Humana, 2016

Inhaltsangabe

This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.

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This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2016
• Sprache: Englisch
• ISBN 10: 1493949004
• ISBN 13: 9781493949007
• Einband: Taschenbuch
• Anzahl der Seiten: 196
• Herausgeber: Bose Jeffrey L.
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland