Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology, Band 1586) - Softcover

Buch 74 von 381: Methods in Molecular Biology
 
9781493983285: Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology, Band 1586)
Softcover
ISBN 10: 1493983288 ISBN 13: 9781493983285
Verlag: Humana, 2018

Inhaltsangabe

This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput  cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.

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This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput  cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.

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Verlag
Humana
Erscheinungsdatum
2018
Sprache
Englisch
ISBN 10 
1493983288
ISBN 13 
9781493983285
Einband
Taschenbuch
Anzahl der Seiten
444
Herausgeber
Burgess-Brown Nicola A.
Kontakt zum Hersteller
preigu GmbH & Co. KG
mail@preigu.de

Lengericher Landstr. 19
Osnabrück
Niedersachsen
49078
Deutschland

Weitere beliebte Ausgaben desselben Titels

9781493968855: Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology, 1586, Band 1586)

Vorgestellte Ausgabe

ISBN 10:  1493968858 ISBN 13:  9781493968855
Verlag: Humana, 2017
Hardcover