Host-Pathogen Interactions: Methods and Protocols: 1734 (Methods in Molecular Biology) - Softcover

Softcover

ISBN 10: 1493985329 ISBN 13: 9781493985326

Verlag: Humana, 2018

Inhaltsangabe

1. Genetic Association Studies in Host-Pathogens Interaction Analysis

Jose Luis Royo and Luis Miguel Real

2. Bacterial Genotyping Methods: From the Basics to Modern

Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, and Doris Gómez-Camargo

Part I: Animal-Pathogen Interaction Section

3. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

Gili Aviv and Ohad Gal-Mor

4. The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection

Gili Aviv and Ohad Gal-Mor

5. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

Gili Aviv and Ohad Gal-Mor

6. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity

Gili Aviv and Ohad Gal-Mor

7. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage

Carlos Medina, Beatriz Mesa-Pereira, Eva Mª Camacho, Amando Flores, and Eduardo Santero

8. Organoids as a Model to Study Infectious Disease

Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, and Eitaro Aihara

9. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins

Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak, and Mangesh Bhide

10. Systems Biology Modeling to Study Pathogen-Host Interactions

Müberra Fatma Cesur and Saliha Durmuş

11. Phage Therapy: Various Perspectives on How to Improve the Art

Stephen T. Abedon

Part II: Microbe-Microbe Interaction Section

12. Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

Cristina Isabel Amador, Claus Sternberg, and Lars Jelsbak

13. Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth

Fernando Govantes

14. Detection of Bacterial Quorum Sensing Molecules

Elke Stein and Adam Schikora

Part III: Plant-Pathogen Interaction Section

15. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae

José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, and Carmen R. Beuzón

16. Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation

Anthony Perrier, Patrick Barberis, and Stephane Genin

17. In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors

Fabien Lonjon, Nemo Peeters, Stéphane Genin, and Fabienne Vailleau

18. Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains

Alice Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthomé, and Alice Guidot

19. Methods to Quantify Biotic-Induced Stress in Plants

Marcel Bach-Pages and Ga

Über diesen Titel

Von der hinteren Coverseite

This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2018
• ISBN 10: 1493985329
• ISBN 13: 9781493985326
• Einband: Tapa blanda
• Sprache: Englisch
• Anzahl der Seiten: 348
• Herausgeber: Medina Carlos, López-Baena Francisco Javier
• Kontakt zum Hersteller: Nicht verfügbar