Two-Hybrid Systems: Methods and Protocols (Methods in Molecular Biology, 1794, Band 1794) - Softcover

 
9781493993109: Two-Hybrid Systems: Methods and Protocols (Methods in Molecular Biology, 1794, Band 1794)
Softcover
ISBN 10: 1493993100 ISBN 13: 9781493993109
Verlag: Humana, 2019

Inhaltsangabe

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab. 


Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

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This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab. 


Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

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Verlag
Humana
Erscheinungsdatum
2019
Sprache
Englisch
ISBN 10 
1493993100
ISBN 13 
9781493993109
Einband
Taschenbuch
Anzahl der Seiten
367
Herausgeber
Oñate-Sánchez Luis
Kontakt zum Hersteller
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Heidelberg
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Deutschland

Weitere beliebte Ausgaben desselben Titels

9780896038325: Two-Hybrid Systems: Methods and Protocols (Methods in Molecular Biology, Band 177)

Vorgestellte Ausgabe

ISBN 10:  0896038327 ISBN 13:  9780896038325
Verlag: Humana, 2001
Hardcover