Trinucleotide Repeats: Methods and Protocols: 2056 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 1493997831 ISBN 13: 9781493997831

Verlag: Springer-Verlag GmbH, 2019

Inhaltsangabe

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

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This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Details zur Ausgabe

• Verlag: Springer-Verlag GmbH
• Erscheinungsdatum: 2019
• Sprache: Englisch
• ISBN 10: 1493997831
• ISBN 13: 9781493997831
• Einband: Tapa dura
• Auflage: 1
• Anzahl der Seiten: 286
• Herausgeber: Richard Guy-Franck
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: CMN Printpool Inh. Mathis Neumann; https://www.also.com/ec/cms5/en_6000/6000/index.jsp; ; Friedrich-Karl-Str. 9; Hamburg; Deutschland