Macrophage Migration Inhibitory Factor: Methods and Protocols: 2080 (Methods in Molecular Biology) - Softcover

 
9781493999385: Macrophage Migration Inhibitory Factor: Methods and Protocols: 2080 (Methods in Molecular Biology)
Softcover
ISBN 10: 1493999389 ISBN 13: 9781493999385
Verlag: Humana, 2020

Inhaltsangabe

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. 


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This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease. 

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Verlag
Humana
Erscheinungsdatum
2020
Sprache
Englisch
ISBN 10 
1493999389
ISBN 13 
9781493999385
Einband
Tapa blanda
Auflage
1
Anzahl der Seiten
280
Herausgeber
Harris James, Morand Eric F.
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
CMN Printpool Inh. Mathis Neumann
https://www.also.com/ec/cms5/en_6000/6000/index.jsp

Friedrich-Karl-Str. 9
Hamburg
Deutschland

Weitere beliebte Ausgaben desselben Titels

9781493999354: Macrophage Migration Inhibitory Factor: Methods and Protocols: 2080 (Methods in Molecular Biology)

Vorgestellte Ausgabe

ISBN 10:  1493999354 ISBN 13:  9781493999354
Verlag: Humana, 2019
Hardcover