Excerpt from An Adaptation of Folin's Method for the Quantitative Determination of Creatine and Creatinine
The quantitative method for the determination of creat ine and creatinine, which was perfected by Folig? Is the most accurate which we possess to-day. This method is based on the werk of Jafféf'who showed that when a solution containing creat inine is treated with a solution of picric acid and made alka line with sodium hydroxide a beafiiful deep,red -orange color is produced. Previous to this time creatinine was precipitated with zinc chloride, filtered, and weighed as the double salt of creat inine and zinc chloride. Folin's method was perfected for the use in the determination of creatinine in urines. The method is as follows: Place ten cubic centimeters of urine in a 500 cc. Volumetric flask, add 15cc. Of a saturated solution of picric acid and Sec. Of a 10 per cent solution of sodium hydroxide; then shake thoroughly, and allow the mixture to stand. After exactly five minutes, dilute the contents of the flask to the 500cc. Mark, and pour some of the solution ants one of the cylinders of the Duboscdrcolorimeter. One of the cylinders of the colorimeter is filled with a half-normal solution of potassium dichromate and the prism plunged into it until the scale reads 8mm. In the one containing the solution of creatinine, picric acid, and sodium hydroxide the prism plunger is adjusted until there is an equal depth of color in the two halves of the field at the eye piece. Four readings should be made in each determination and the average used as the final result. From such data, the amount.
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Paperback. Zustand: New. Print on Demand. This book presents an in-depth exploration of an advanced variation of Folin's method, one of the most accurate quantitative methods for determining creatinine and creatine, substances found in urine that provide critical insights into muscle metabolism. The author delves into the nuances of the original method, highlighting its strengths and exploring areas for improvement. The refined technique extends the applicability of the method to instances where only minute quantities of creatine are available, offering greater precision in various research and clinical settings. Through experimentation, the author examines the factors that influence the rate of creatine extraction from muscles, providing valuable insights into the underlying mechanisms and the impact of factors such as muscle structure and the nature of the extraction medium. This book is an indispensable resource for researchers and professionals seeking a deeper understanding and mastery of the Folin method for determining creatine and creatinine, offering a comprehensive examination of the technique and its diverse applications. This book is a reproduction of an important historical work, digitally reconstructed using state-of-the-art technology to preserve the original format. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in the book. print-on-demand item. Bestandsnummer des Verkäufers 9781528528559_0
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PAP. Zustand: New. New Book. Shipped from UK. Established seller since 2000. Bestandsnummer des Verkäufers LX-9781528528559
Anbieter: PBShop.store UK, Fairford, GLOS, Vereinigtes Königreich
PAP. Zustand: New. New Book. Shipped from UK. Established seller since 2000. Bestandsnummer des Verkäufers LX-9781528528559
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