Handbook of ELISPOT: Methods and Protocols: v. 302 (Methods in Molecular Biology) - Hardcover

 
9781588294692: Handbook of ELISPOT: Methods and Protocols: v. 302 (Methods in Molecular Biology)
Hardcover
ISBN 10: 1588294692 ISBN 13: 9781588294692
Verlag: Humana Press Inc., 2005

Inhaltsangabe

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

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Críticas

From the reviews: "The book is divided into four sections, with a total of 20 chapters featuring step-by-step protocols that will enable both novice and experienced researchers to perform ELISPOT. Each chapter begins with a general Introduction, followed by a detailed Materials section and an easy-to-follow Methods section ... . All the chapters are equally interesting and well presented ... . a must for a cytochemist. This book will serve as a convenient reference manual for beginners and for experienced ELISPOT users." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)

Reseña del editor

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

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Verlag
Humana Press Inc.
Erscheinungsdatum
2005
Sprache
Englisch
ISBN 10 
1588294692
ISBN 13 
9781588294692
Einband
Tapa dura
Anzahl der Seiten
336
Herausgeber
Kalyuzhny Alexander E.
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Nicht verfügbar

Weitere beliebte Ausgaben desselben Titels

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Vorgestellte Ausgabe

ISBN 10:  1617375888 ISBN 13:  9781617375880
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Softcover