This book is a comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful "Methods in Molecular Biology" series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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From the reviews: "The book presents 22 chapters from an international group of experts in the field a ] . Human Embryonic Stem Cell Protocols is designed to provide a collection of the essential methods in hESC culture and maintenance a ] . a comprehensive set of valuable protocols from leading research labs in the world. a ] This book is a valuable resource for hESC investigators who hope to expand their understanding of the manipulation of hESCs." (Elayne Chan and George Q. Daley, Bone Marrow Transplantation, May, 2006)
A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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