Neurotransmitter Methods (Methods in Molecular Biology, Band 72) - Softcover

Softcover

ISBN 10: 1617370231 ISBN 13: 9781617370236

Verlag: Humana, 2010

Inhaltsangabe

Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action. Each method is detailed in a user-friendly "recipe" format and the protocols are accompanied by extensive notes to high light and explain crucial steps. Approaches utilizing an incredibly diverse array of modem techniques are presented: methods including HPLC, histochemistry, immunocytochemistry, in situ hybridization, mass spectrometry, microdialysis, and electrochemistry all make at least one appearance. In addition, protocols for associated methodologies, including the production of brain slices, dissociated neurons, synaptosomes/synaptoneurosomes, and neuronal plasma membranes are presented. Methods applicable to most of the recognized chemical types of neu rotransmitter are to be found and, although you may find absent any mention of your favorite neurotransmitter, many of the protocols are sufficiently general to be adapted to alternative uses. So, how does this book contribute usefiilly to the horde of methods volumes stampeding across our bookshelves? 1 hope that the strength oiNeurotransmitter Methods lies in the variety of its content. The book provides in a single volume an array of techniques that could take a researcher from selection and preparation of a tissue source through to identification and measurement of neurotransmitter content and even onto characterization of neurotransmitter sites of action. Furthermore, the diversity of approaches presented reflects the need for today's bench neuroscientist to be a bit of a "jack (or a "jill"!) of all trades.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2010
• Sprache: Englisch
• ISBN 10: 1617370231
• ISBN 13: 9781617370236
• Einband: Taschenbuch
• Anzahl der Seiten: 284
• Herausgeber: Rayne Richard C.
• Kontakt zum Hersteller: preigu GmbH & Co. KG; mail@preigu.de; ; Lengericher Landstr. 19; Osnabrück; Niedersachsen; 49078; Deutschland