Confocal Microscopy: Methods and Protocols (Methods in Molecular Biology, Band 122) - Softcover

Softcover

ISBN 10: 1617370622 ISBN 13: 9781617370625

Verlag: Humana, 2010

Inhaltsangabe

An Introduction to Confocal Imaging.- Practical Considerations for Collecting Confocal Images.- Fluorescent Probes for Confocal Microscopy.- Imaging Gene Expression Using Antibody Probes.- Single and Double FISH Protocols for Drosophila.- Confocal Microscopy of Plant Cells.- Preparation of Yeast Cells for Confocal Microscopy.- Confocal Methods for Caenorhabditis elegans.- Imaging Sea Urchin Fertilization.- Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy.- Confocal Microscopy on Xenopus laevis Oocytes and Embryos.- Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos.- Confocal Imaging of Living Cells in Intact Embryos.- Live Confocal Analysis with Fluorescently Labeled Proteins.- Live Imaging with Green Fluorescent Protein.- Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging.- Intracellular pH and pCa Measurement.- Measuring Dynamic Cell Volume In Situ by Confocal Microscopy.- Imaging Thick Tissues with Confocal Microscopy.- Measurement in the Confocal Microscope.- Presentation of Confocal Images.- The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides.- Information Management of Confocal Microscopy Images.- Morphing Confocal Images and Digital Movie Production.

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In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish.

Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2010
• Sprache: Englisch
• ISBN 10: 1617370622
• ISBN 13: 9781617370625
• Einband: Taschenbuch
• Anzahl der Seiten: 464
• Herausgeber: Paddock Stephen W.
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland