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Pichia Protocols (Methods in Molecular Biology, Band 389) - Softcover

Buch 44 von 381: Methods in Molecular Biology
 
9781617375637: Pichia Protocols (Methods in Molecular Biology, Band 389)
Softcover
ISBN 10: 1617375632 ISBN 13: 9781617375637
Verlag: Humana, 2010

Inhaltsangabe

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).

Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.

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Von der hinteren Coverseite

The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system

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Verlag
Humana
Erscheinungsdatum
2010
Sprache
Englisch
ISBN 10 
1617375632
ISBN 13 
9781617375637
Einband
Taschenbuch
Auflage
2
Anzahl der Seiten
280
Herausgeber
Cregg James M.
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