SARS- and Other Coronaviruses: Laboratory Protocols (Methods in Molecular Biology, Band 454) - Softcover

Softcover

ISBN 10: 1617378151 ISBN 13: 9781617378157

Verlag: Humana, 2010

Inhaltsangabe

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In this book, expert researchers examine these devastating viruses through state-of-the-art, widely applicable protocols with minute detail. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Comprehensive and cutting-edge, this book serves as an ideal guide for all virologists and especially for those working with coronaviruses.

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In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2010
• Sprache: Englisch
• ISBN 10: 1617378151
• ISBN 13: 9781617378157
• Einband: Taschenbuch
• Anzahl der Seiten: 360
• Herausgeber: Cavanagh Dave
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland