Signal Transduction Immunohistochemistry: Methods and Protocols (Methods in Molecular Biology, 717, Band 717) - Hardcover

Hardcover

ISBN 10: 1617790230 ISBN 13: 9781617790232

Verlag: Humana, 2011

Inhaltsangabe

Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.  In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC).  Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

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Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2011
• Sprache: Englisch
• ISBN 10: 1617790230
• ISBN 13: 9781617790232
• Einband: Gebundene Ausgabe
• Anzahl der Seiten: 315
• Herausgeber: Kalyuzhny Alexander E.
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland