Cell Cycle Synchronization: Methods and Protocols: 761 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 1617791814 ISBN 13: 9781617791819

Verlag: Humana Press, 2011

Inhaltsangabe

1. Overview of Cell Synchronization

Gaspar Banfalvi

2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

Gaspar Banfalvi

3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer

4. Large Scale Mitotic Cell Synchronization

Kalyan Dulla and Anna Santamaria

5. Synchronization of Mammalian Cell Cultures by Serum Deprivation

Thomas J. Langan and Richard C. Chou

6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry

Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka

7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes

Takashi Iwamatsu

8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis

Congjun Li

9. Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover during the Cell Cycle

Jennifer A. Fischer and Salvatore Caradonna

10. Synchronization of HeLa Cells

Hoi Tang Ma and Randy Y.C. Poon

11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

Gaspar Banfalvi

12. Synchronization of Yeast

Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider

13. Synchronization of Pathogenic Protozoans

Staffan Svärd and Karin Troell

14. Synchronization of In Vitro Maturation in Porcine Oocytes

Tamas Somfai and Yuji Hirao

15. Synchronization of Medicago sativa Cell Suspension Culture

Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth

16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)

Yoel Shufaro and Benjamin E. Reubinoff

17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

Kap-Hyoun Ko, Robert Nordon, Tracey A. O’Brien, Geoff Symonds, and Alla Dolnikov

18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients

Fabienne Venet, Caroline Guignant, and Guillaume Monneret

19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish

Attila Csikász-Nagy, Alida Palmisano, and Judit Zámborszky

Über diesen Titel

Über die Autorin bzw. den Autor

Charlotte y Peter Fiell son dos autoridades en historia, teoría y crítica del diseño y han escrito más de sesenta libros sobre la materia, muchos de los cuales se han convertido en éxitos de ventas. También han impartido conferencias y cursos como profesores invitados, han comisariado exposiciones y asesorado a fabricantes, museos, salas de subastas y grandes coleccionistas privados de todo el mundo. Los Fiell han escrito numerosos libros para TASCHEN, entre los que se incluyen 1000 Chairs, Diseño del siglo XX, El diseño industrial de la A a la Z, Scandinavian Design y Diseño del siglo XXI.

Von der hinteren Coverseite

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the arrest-and-release approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Details zur Ausgabe

• Verlag: Humana Press
• Erscheinungsdatum: 2011
• Sprache: Englisch
• ISBN 10: 1617791814
• ISBN 13: 9781617791819
• Einband: Tapa dura
• Anzahl der Seiten: 308
• Herausgeber: Banfalvi Gaspar
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Nicht verfügbar