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Free Radicals and Antioxidant Protocols: Second Edition: 610 (Methods in Molecular Biology) - Softcover
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This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers.
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In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike.
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Free Radicals and Antioxidant Protocols (Methods in Molecular Biology, 610)
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Free Radicals and Antioxidant Protocols (Paperback)
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Paperback. Zustand: new. Paperback. Reactive oxygen and nitrogen species (RONs) affect normal physiological processes and pathological conditions. In spite of the development of analytical and technological advancements in the detection and determination of RONs, there is a growing demand to achieve more rapid and accurate detection and determination of RONs and also of redox stress. In order to satisfy such a demand, we chose to prepare this current volume which comprises 27 chapters contributed by world-renowned experts on the state-- the-art analytical and technological aspects of the detection and determination of RONs, and oxidative, nitrative, nitrosative, and redox stresses in biological systems, in vitro and in vivo. There is a need to establish biomarkers for the oxidative stress-induced genetic d- age in cellular and animal models. This need is addressed herein by offering protocols for (1) the generation of stable oxidative stress-resistant phenotypes of Chinese hamsters ?broblasts, (2) in vivo detection and measurement of free radicals and oxygen, (3) in vivo determination of tissue and DNA damage as a result of radical exposure, and (4) in vivo and in vitro monitoring of footprints of free-radical and antioxidant reactions.In the s- tions dedicated to the analysis of antioxidants and their metabolites, we describe methods for the analysis of phenolic acids and ?avonoids, eugenol antioxidants, and the recycling of ascorbic and lipoic acids. We also report on cellular reductive capacity, determination of glutathione, mitochondrial transmembrane potential, and cytotoxicity in cardiomyocytes under RONs stresses. This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Bestandsnummer des Verkäufers 9781617796838
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Free Radicals and Antioxidant Protocols (Methods in Molecular Biology, 610)
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Free Radicals and Antioxidant Protocols
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Zustand: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Comprised of twenty-seven chapters contributed by world-renowned experts in the fieldFully updated from the first edition to offer cutting-edge methods for the rapid detection of RONs and redox stressIncludes in-depth analysis of natural an. Bestandsnummer des Verkäufers 4259228
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Free Radicals and Antioxidant Protocols
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Taschenbuch. Zustand: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike. 496 pp. Englisch. Bestandsnummer des Verkäufers 9781617796838
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Free Radicals and Antioxidant Protocols
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Zustand: New. This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers. Editor(s): Uppu, Rao M.; Murthy, Subramanyam N.; Pryor, William A.; Parinandi, Narasimham L. Series: Methods in Molecular Biology. Num Pages: 496 pages, 104 black & white illustrations, 11 colour illustrations, 19 black & white tables, biogra. BIC Classification: PNF; PSB; PSF. Category: (P) Professional & Vocational. Dimension: 254 x 178 x 25. Weight in Grams: 935. . 2012. Softcover reprint of hardcover 2nd ed. 2010. Paperback. . . . . Bestandsnummer des Verkäufers V9781617796838
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Taschenbuch. Zustand: Neu. Neuware -Reactive oxygen and nitrogen species (RONs) affect normal physiological processes and pathological conditions. In spite of the development of analytical and technological advancements in the detection and determination of RONs, there is a growing demand to achieve more rapid and accurate detection and determination of RONs and also of redox stress. In order to satisfy such a demand, we chose to prepare this current volume which comprises 27 chapters contributed by world-renowned experts on the state-- the-art analytical and technological aspects of the detection and determination of RONs, and oxidative, nitrative, nitrosative, and redox stresses in biological systems, in vitro and in vivo. There is a need to establish biomarkers for the oxidative stress-induced genetic d- age in cellular and animal models. This need is addressed herein by offering protocols for (1) the generation of stable oxidative stress-resistant phenotypes of Chinese hamsters broblasts, (2) in vivo detection and measurement of free radicals and oxygen, (3) in vivo determination of tissue and DNA damage as a result of radical exposure, and (4) in vivo and in vitro monitoring of footprints of free-radical and antioxidant reactions. In the s- tions dedicated to the analysis of antioxidants and their metabolites, we describe methods for the analysis of phenolic acids and avonoids, eugenol antioxidants, and the recycling of ascorbic and lipoic acids. We also report on cellular reductive capacity, determination of glutathione, mitochondrial transmembrane potential, and cytotoxicity in cardiomyocytes under RONs stresses.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 496 pp. Englisch. Bestandsnummer des Verkäufers 9781617796838
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Taschenbuch. Zustand: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - In recent years there has been a growing demand to achieve more rapid and accurate detection of RONs (reactive oxygen and nitrogen species), which affect normal physiological processes and pathological conditions, and also of redox stress. In Free Radicals and Antioxidant Protocols, Second Edition, world-renowned researchers respond to this need, considering the most recent analytical and technological aspects of the detection and determination of RONs, and of oxidative, nitrative, nitrosative, and redox stress in biological systems in vivo and in vitro. Chapters include protocols to establish biomarkers for the oxidative stress-induced genetic damage in cellular and animal models, provide novel methods and technologies for analyzing peroxidized lipids, describe techniques for gene therapy, and present methods of EPR imaging of free radicals and oxygen in vivo. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and user-friendly, Free Radicals and Antioxidant Protocols, Second Edition, is an essential guide for the novice and expert researcher alike. Bestandsnummer des Verkäufers 9781617796838
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Free Radicals and Antioxidant Protocols
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Free Radicals and Antioxidant Protocols
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Zustand: New. This cutting-edge and updated book offers methods for the rapid detection of RONs and redox stress. It includes in-depth analysis of natural and synthetic antioxidants, and also of DNA oxidation, oxidative lipidomics, and biomarkers. Editor(s): Uppu, Rao M.; Murthy, Subramanyam N.; Pryor, William A.; Parinandi, Narasimham L. Series: Methods in Molecular Biology. Num Pages: 496 pages, 104 black & white illustrations, 11 colour illustrations, 19 black & white tables, biogra. BIC Classification: PNF; PSB; PSF. Category: (P) Professional & Vocational. Dimension: 254 x 178 x 25. Weight in Grams: 935. . 2012. Softcover reprint of hardcover 2nd ed. 2010. Paperback. . . . . Books ship from the US and Ireland. Bestandsnummer des Verkäufers V9781617796838
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