Methods in Yeast Genetics and Genomics: A Cold Spring Harbor Laboratory Course Manual, 2015 Edition - Softcover

Dunham, Maitreya J

Softcover

ISBN 10: 162182134X ISBN 13: 9781621821342

Verlag: Cold Spring Harbor Laboratory Press, 2015

Inhaltsangabe

Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2005), revolutionary advances in genomics, proteomics, and imaging technologies have had a significant impact on the field. The 11 experiments included in this manual provide a foundation of methods for any modern-day yeast lab. These methods emphasize combinations of classical and modern genetic approaches, including isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, complementation, and recombination. Also covered are molecular genetic techniques for genome engineering. Additional experiments introduce fundamental techniques in yeast genomics, including both performance and interpretation of Synthetic Genetic Array analysis, multiplexed whole genome and barcode sequencing, and comparative genomic hybridization to DNA arrays. Comparative genomics is introduced using different yeast strains to study natural variation, evolution, and quantitative traits. This manual covers the full repertoire of genetic approaches needed to dissect complex biological problems in the yeast Saccharomyces cerevisiae.

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Reseña del editor

The Yeast Genetics & Genomics course is a modern, state of the art, laboratory course designed to teach the full repertoire of genetic approaches needed to dissect complex problems in the yeastSaccharomyces cerevisiae. The course is designed for researchers who wish to use budding yeast as a model to study multiple cellular processes. The curriculum for this course embodies three components: (1) a rigorous and comprehensive set of laboratory experiments; (2) a series of detailed theoretical lectures by the instructors describing current knowledge, concepts, techniques, and strategies employed in yeast genetics & genomics research; and (3) a series of seminars by invited speakers describing their research, with an emphasis on unique and imaginative approaches at the forefront of the yeast genetics & genomics field.

Details zur Ausgabe

• Verlag: Cold Spring Harbor Laboratory Press
• Erscheinungsdatum: 2015
• Sprache: Englisch
• ISBN 10: 162182134X
• ISBN 13: 9781621821342
• Einband: Tapa blanda
• Anzahl der Seiten: 252
• Herausgeber: Gartenberg Marc R, Brown Grant W
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Nicht verfügbar