Calcium Signaling Protocols (Methods in Molecular Biology, 937, Band 937) - Hardcover

Hardcover

ISBN 10: 1627030859 ISBN 13: 9781627030854

Verlag: Humana, 2012

Inhaltsangabe

The regulation of intracellular Ca²⁺ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca²⁺ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca²⁺ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca²⁺.

Über diesen Titel

Von der hinteren Coverseite

The regulation of intracellular Ca²⁺ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca²⁺ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca²⁺ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca²⁺.

Details zur Ausgabe

• Verlag: Humana
• Erscheinungsdatum: 2012
• Sprache: Englisch
• ISBN 10: 1627030859
• ISBN 13: 9781627030854
• Einband: Gebundene Ausgabe
• Auflage: 3
• Anzahl der Seiten: 371
• Herausgeber: Lambert David G., Rainbow Richard D.
• Kontakt zum Hersteller: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Deutschland