Metalloproteins: Methods and Protocols: 1122 (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 1627037934 ISBN 13: 9781627037938

Verlag: Humana, 2014

Inhaltsangabe

<P>METALLOPROTEINS ARE INVOLVED IN MANY KEY BIOLOGICAL PROCESSES SUCH AS GAS TRANSPORT AND METABOLISM, PHOTOSYNTHESIS, CELL RESPIRATION, THE KREBS CYCLE AND MANY OTHER VITAL REDOX REACTIONS. <I>METALLOPROTEINS: METHODS AND PROTOCOLS</I> ADDRESSES MULTIPLE ASPECTS OF METALLOENZYME RESEARCH FROM THE PRODUCTION AND PURIFICATION OF METALLOPROTEINS USING BOTH STANDARD RECOMBINANT TECHNIQUES AND A CELL-FREE SYSTEM, TO THE ELECTROCHEMICAL ANALYSIS OF METALLOPROTEINS, AS WELL AS IR TECHNIQUES, MÖSSBAUER SPECTROSCOPY AND THE THEORETICAL INTERPRETATION OF METALLOENZYME CATALYTIC AND REDOX PROCESSES, TO NAME A FEW TOPICS. WRITTEN IN THE SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE PROTOCOLS AND NOTES ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><P>AUTHORITATIVE AND EASILY ACCESSIBLE, <I>METALLOPROTEINS: METHODS AND PROTOCOLS</I> SEEKS TO SERVE BOTH PROFESSIONALS AND NOVICES INTERESTED IN THE METALLOPROTEIN FIELD. </P>

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Reseña del editor:

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

Über diese Auflage

• Verlag: Humana
• Erscheinungsdatum: 2014
• ISBN 10: 1627037934
• ISBN 13: 9781627037938
• Einband: Tapa dura
• Anzahl der Seiten: 312
• Herausgeber: Fontecilla-Camps Juan C., Nicolet Yvain