Gene Targeting and Embryonic Stem Cells (Advanced Methods) - Softcover

Thomson, Alison

Softcover

ISBN 10: 1859963609 ISBN 13: 9781859963609

Verlag: Bios Scientific Publishers Ltd, 2004

Inhaltsangabe

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines.  Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.

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Über die Autorin bzw. den Autor

Jim McWhir (The Roslin Institute, UK) (Author) ,  Alison Thomson (The Roslin Institute, UK) (Author)

Details zur Ausgabe

• Verlag: Bios Scientific Publishers Ltd
• Erscheinungsdatum: 2004
• Sprache: Englisch
• ISBN 10: 1859963609
• ISBN 13: 9781859963609
• Einband: Taschenbuch
• Anzahl der Seiten: 205
• Herausgeber: Mcwhir Jim
• Kontakt zum Hersteller: Nicht verfügbar
• Verantwortliche Person: Logos Europe; contact@logoseurope.eu; ; Rue Nicolas Possin 9; La Rochelle; 17000; Frankreich