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This fourth edition of "The Mouse Manual¨is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on:
· generation of induced pluripotent stem cells
· RNA microinjections
· lentiviral microinjections and infection
· assisted reproduction techniques for sperm and embryo cryopreservation
· isolation, generation, and transplantation of spermatogonial stem cell lines
· in utero electroporation of gene constructs into postimplantation embryos
· vibratome sectioning of live and fixed tissues for imaging thick tissue sections
· whole-mount fluorescent staining methods for three-dimensional visualization
Recombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field's technological sophistication has grown exponentially but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.
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Charlotte y Peter Fiell son dos autoridades en historia, teoría y crítica del diseño y han escrito más de sesenta libros sobre la materia, muchos de los cuales se han convertido en éxitos de ventas. También han impartido conferencias y cursos como profesores invitados, han comisariado exposiciones y asesorado a fabricantes, museos, salas de subastas y grandes coleccionistas privados de todo el mundo. Los Fiell han escrito numerosos libros para TASCHEN, entre los que se incluyen 1000 Chairs, Diseño del siglo XX, El diseño industrial de la A a la Z, Scandinavian Design y Diseño del siglo XXI.
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Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition
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Cold Spring Harbor Laboratory Press, 2013
ISBN 10: 1936113015
ISBN 13: 9781936113019
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Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition
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Cold Spring Harbor Laboratory Press,U.S., 2014
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Manipulating the Mouse Embryo : A Laboratory Manual
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Manipulating the Mouse Embryo : A Laboratory Manual
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ISBN 13: 9781936113019
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Manipulating the Mouse Embryo : A Laboratory Manual
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Cold Spring Harbor Laboratory Press, 2013
ISBN 10: 1936113015
ISBN 13: 9781936113019
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Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition
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Cold Spring Harbor Laboratory Press,U.S., 2013
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Taschenbuch. Zustand: Neu. Neuware - This 4th edition of the 'Mouse Manual'-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on: - generation of induced pluripotent stem cells RNA microinjections lentiviral microinjections and infection assisted reproduction techniques for sperm and embryo cryopreservation isolation, generation, and transplantation of spermatogonial stem cell lines in utero electroporation of gene constructs into postimplantation embryos vibratome sectioning of live and fixed tissues for imaging thick tissue sections whole-mount fluorescent staining methods for three-dimensional visualization In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice. I have the third edition. Why should I buy the fourth edition The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the field and offer clear step-by-step guidance with copious illustrations. To whom is this edition addressed-the neophyte or the experienced investigator Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction to the field. For the experienced investigator, the detailed protocols of new techniques in the field, with extensive troubleshooting and over-the-shoulder advice, allow easy integration of these techniques into a laboratory's research objectives.Are the protocols in this book better than those available for download on the Web Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have. In the case of the protocols in this manual, there is a bit of 'art' required in the performance of these protocols. But the experience and advice of these authors provides all of the information needed to achieve success in the lab. Bestandsnummer des Verkäufers 9781936113019
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