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Preface
Foreword
Part 1. Litter dynamics
1. Litter Input (Arturo Elosegi & Jesús Pozo)
2. Leaf Retention (Arturo Elosegi)
3. Manipulating Litter Retention in Streams (Michael Dobson)
4. Coarse Benthic Organic Matter (Jesús Pozo & Arturo Elosegi)
5. Leaching (Felix Bärlocher)
6. Leaf Mass Loss Estimated by the Litter-Bag Technique (Felix Bärlocher)
7. Determining Litter Mass Loss by the Plant Tagging Approach (Kevin A. Kuehn & Mark O. Gessner)
8. Wood Decomposition (Arturo Elosegi, Maite Arroita & Libe Solagaistua)
9. Decomposition of Fine Particulate Organic Matter (Yoshimura Chihiro)
10. Coarse Particulate Organic Matter Budgets (Jesús Pozo & Jon Molinero)
Part 2. Chemical and Physical Leaf Properties
11. Total Phosphorus, Nitrogen, and Carbon in Leaf Litter (Mogens R. Flindt, Ana I. Lillebø, Javier Pérez & Verónica Ferreira)
12. Total Protein (Mark O. Baerlocher)
13. Free Amino Acids (Shawn D. Mansfield & Mark O. Baerlocher)
14. Determination of Total Carbohydrates (Shawn D. Mansfield)
15. Determination of Soluble Carbohydrates (Letitia da Ros, Faride Unda, Shawn D. Mansfield)
16. Total Lipids (Mark O. Gessner & Paul T. M. Neumann)
17. Polyunsaturated Fatty Acids in Decomposing Leaf Litter (Eric Von Elert)
18. Total Phenolics (Felix Bärlocher & Manuel A.S. Graça)
19. Radial Diffusion Assay for Tannins (Manuel A.S. Graça & Felix Bärlocher)
20. Acid Butanol Assay to Determine Bulk Concentrations of Condensed Tannins (Mark O. Gessner & Daniel Steiner)
21. Lignin and Cellulose (Mark O. Gessner)
22. Physical Litter Properties: Leaf Toughness and Tensile Strength (Manuel A.S. Graça & Martin Zimmer)
Part 3. Microbial Decomposers
23. Techniques for Handling Ingoldian Fungi (Enrique Descals)
24. Maintenance of Aquatic Hyphomycete Cultures (Ludmila Marvanová)
25. An Illustrated Key to the Common Temperate Species of Aquatic Hyphomycetes (Vladislav Gulis, Ludmila Marvanová & Enrique Descals)
26. Sporulation by Aquatic Hyphomycetes (Felix Bärlocher)
27. Ergosterol as a Measure of Fungal Biomass (Mark O. Gessner)
28. Fungal Growth Rates and Production (Keller Suberkropp, Mark O. Gessner & Kevin A. Kuehn)
29. Bacterial Abundance and Biomass Determination in Plant Litter by Epifluorescence Microscopy (Nanna Buesing & Mark O. Gessner)
30. Growth and Production of Litter-Associated Bacteria (Nanna Buesing, Mark O. Gessner & Kevin A. Kuehn)
31. Isolation of Cellulose-Degrading Bacteria (Jürgen Marxsen)
32. ATP as a Measure of Microbial Biomass (Manuela Abelho)
33. Respiration of Litter-Associated Microbes and Invertebrates (Manuel A.S. Graça & Manuela Abelho)
Part 4. Molecular Microbial Community Analyses
34. Terminal Restriction Fragment Length Polymorphism (T-Rflp) to Estimate Fungal Diversity (Liliya G. Nikolcheva & Felix Bärlocher)
35. Denaturing Gradient Gel Electrophoresis (DGGE) to Estimate Fungal Diversity (Liliya G. Nikolcheva & Felix Bärlocher)
36. Quantitative Real-Time PCR (qPCR) to Estimate Molecular Fungal Abundance (Christiane Baschien & J. Steffen C. Carl)37. Metabarcoding of Litter-associated Fungi and Bacteria (Sofia Duarte, Christian W
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